The regarding “anaerobic” assessment (i.e., non-mitochondrial metabolic pathways) seems to be affected by the exercise modality. The purpose of this study was to verify whether the exercise modality (i.e., running and cycling) alters the magnitude of “anaerobic” capacity estimated by a single supramaximal exhaustive effort (MAODALT). Fourteen healthy men (age: 26±9 years) underwent a maximum incremental test to determine the maximal oxygen uptake (VO2max); the minimal velocity (for the treadmill) and power output (for the cycle ergometer) at which VO2max was reached (iVO2max), was considered iVO2max for each ergometer. A supramaximal effort to exhaustion at 115% of the iVO2max was conducted to determine the MAODALT (i.e., sum of both oxygen equivalents from the glycolytic and phosphagen pathways). During all tests, the respiratory responses were measured breath-by-breath using a stationary gas analyzer (Quark PFT/COSMED Italy). The blood samples were collected in the rest and 3, 5, and 7 min after each effort and the highest [La-] measured was assumed as the peak value for each test. Afterward, it was conducted an electrochemical analysis of lactate (YSI 2300 STAT/Yellow Spring Inst., USA). The VO2max during running was higher (p=0.001; large effect size) vs. cycling (48.9±3.9 mL·kg·min-1 vs. 44.8±5.5mL·kg·min-1 respectively). Contrarily, the oxygen equivalent from the glycolytic metabolism was not different between exercise modalities (p=0.133; small effect size; running=2.35±0.48 L and cycling=2.18±0.58 L). Furthermore, the “anaerobic” capacity was likely meaningfully (3.65±0.70 L) and very likely meaningfully (949.1±5.7 mL·kg-1) greater in running than cycling (3.81±0.71 L and 52.0±8.1 mL·kg-1). Additionally, the contribution of the phosphagen metabolism was higher (p=0.001; large effect size) for running compared to cycling (1.6±0.3 L vs. 1.3±0.3 L respectively). Therefore, the “anaerobic” capacity estimated by the sum of both oxygen equivalents from the glycolytic and phosphagen pathways during a supramaximal effort is influenced by exercise modality and is able to identify the difference in phosphagen metabolic contribution. Acknowledgement: FAPESP Project 2013/12940-8. E-mail: pauloredkva@hotmail.com