The purpose of the study was to investigate the sensitivity of an alternative maximal accumulated oxygen deficit (MAODALT) to discriminate the “anaerobic” capacity while comparing: least trained subjects (LT; n=12), moderately trained subjects (MT; n=12), endurance trained subjects (ET; n=16), and rugby players (RG; n=11). Subjects underwent a graded exercise test on a treadmill and a supramaximal effort at 115% of intensity associated to maximal oxygen uptake for assessing MAODALT. MAODALT was calculated as the sum of oxygen equivalents from the phosphagen (i.e., assumed as the fast component of excess post-exercise oxygen consumption) and glycolytic metabolic (i.e., estimated by subtracting resting blood lactate concentration from peak post-exercise blood lactate concentration, considering a value of 1 mmol·L-1 to be equivalent to 3 mL O2·kg-1 body mass) pathways. MAODALT was significantly higher (P