GALANTAMINE MICROPARTICLES REDUCE INFLAMMATION AND OXIDATIVE STRESS AFTER ACUTE SPINAL CORD INJURY IN RATS
"2022-02-21" // app/Providers/../Base/Publico/Artigo/resources/show_includes/info_artigo.blade.php
App\Base\Administrativo\Model\Artigo {#1843 // app/Providers/../Base/Publico/Artigo/resources/show_includes/info_artigo.blade.php #connection: "mysql" +table: "artigo" #primaryKey: "id" #keyType: "int" +incrementing: true #with: [] #withCount: [] +preventsLazyLoading: false #perPage: 15 +exists: true +wasRecentlyCreated: false #escapeWhenCastingToString: false #attributes: array:35 [ "id" => 83363 "edicao_id" => 195 "trabalho_id" => 88 "inscrito_id" => 217 "titulo" => "GALANTAMINE MICROPARTICLES REDUCE INFLAMMATION AND OXIDATIVE STRESS AFTER ACUTE SPINAL CORD INJURY IN RATS" "resumo" => """ SPINAL CORD INJURY (SCI) IS A DEBILITATING CONDITION WITH NO EFFECTIVE TREATMENT. ONE OF THE GREATEST DIFFICULTIES FOR TREATMENT ARISES FROM THE INFLAMMATION PROCESS. PREVIOUS STUDIES FROM OUR GROUP HAVE INDICATED THAT GALANTAMINE IMPROVES RECOVERY AFTER SCI, AND LITERATURE SHOWS ITS ANTI-INFLAMMATORY AND ANTIOXIDANT EFFECTS, AS WELL AS ITS NEUROPROTECTIVE PROPERTIES. POLYMERIC NANOPARTICLES, DUE TO THEIR DIMINISHED SIDE EFFECTS AND IMPROVED DRUG RELEASE CHARACTERISTICS, PRESENT A GOOD POSSIBILITY FOR TREATMENT. THIS STUDY AIMS TO USE NANOPARTICLES LOADED WITH GALANTAMINE TO MITIGATE SCI CONSEQUENCES IN TERMS OF INFLAMMATION AND OXIDATIVE STRESS. THE MICROPARTICLES WERE ELECTROSPUN WITH 2.5% OF GALANTAMINE IN A 4% PLGA SOLUTION OR 4% PLGA ALONE. THE \r\n DIAMETER AND ZETA POTENTIAL OF THE PARTICLES WERE MEASURED BY DYNAMIC LIGHT SCATTERING. WISTAR RATS WERE SUBMITTED TO A CONTUSION INJURY AT THE THORACIC SPINAL CORD (CEUA 35781). THE ANIMALS WERE DIVIDED INTO 5 GROUPS: LAMINECTOMY; SCI; SCI WITH GALANTAMINE; SCI WITH PLGA PARTICLES; AND SCI WITH PLGA-GALANTAMINE PARTICLES (PG). THREE DAYS AFTER THE INJURY, THE ANIMALS WERE EUTHANIZED AND THE SPINAL CORDS WERE COLLECTED. THE LEVELS OF IL-1β AND IL-6 WERE EVALUATED BY ELISA. THE PRODUCTION OF REACTIVE OXYGEN SPECIES (ROS) WAS ASSESSED BY MEASURING THE OXIDATION OF 2€², 7€²-DICHLOROFLUORESCIN DIACETATE (DCF) AND LEVELS OF LIPID PEROXIDATION WERE ANALYZED BY MEASURING THIOBARBITURIC ACID REACTIVE SUBSTANCES (TBARS). THE AVERAGE PARTICLE DIAMETER WAS 3247,6±1490,4NM FOR THE 4% PLGA PARTICLES AND 568,3±199,2NM FOR THE PG PARTICLES. THE ZETA POTENTIAL OF THE PARTICLES WAS -50,05±11.89MV FOR THE 4% PLGA PARTICLES AND -23,59±5.27MV FOR THE PG PARTICLES. THE GROUP TREATED WITH PG SHOWED SIGNIFICANTLY DECREASED IL-1β LEVELS WHEN COMPARED TO THE INJURY GROUP. INTERESTINGLY, THE PLGA GROUP PRESENTED DECREASED IL-6 LEVELS COMPARED TO THE INJURY AND LAMINECTOMY GROUPS. HOWEVER, ONLY THE ANIMALS TREATED WITH PG SHOWED DECREASED ROS PRODUCTION, WHEREAS THE PG AND GALANTAMINE GROUPS SHOWED DECREASED LEVELS OF LIPID PEROXIDATION. THUS, THE USE OF PG PARTICLES IS A PROMISING APPROACH FOR SPINAL CORD INJURY REGENERATION, CONSIDERING ITS EFFECTS IN BOTH THE DECREASE OF INFLAMMATORY CYTOKINES AND OXIDATIVE STRESS MARKERS. """ "modalidade" => "Poster" "area_tematica" => "NANOTECHNOLOGY IN BIOMATERIALS" "palavra_chave" => "SPINAL CORD INJURY, NANOTECHNOLOGY, GALANTAMINE, , " "idioma" => "Português" "arquivo" => "TRABALHO_EV170_MD4_SA_ID217_07122021114225.pdf" "created_at" => "2022-02-16 10:17:52" "updated_at" => null "ativo" => 1 "autor_nome" => "FERNANDA STAPENHORST FRANÇA" "autor_nome_curto" => "STAPENHORST FRANÇA" "autor_email" => "fernanda.stapenhorst@gmai" "autor_ies" => "UFRGS" "autor_imagem" => "" "edicao_url" => "anais-do-latin-american-congress-of-artificial-organs-and-biomaterials" "edicao_nome" => "Anais do XI Latin American Congress of Artificial Organs and Biomaterials (COLAOB)" "edicao_evento" => "XI Latin American Congress of Artificial Organs and Biomaterials" "edicao_ano" => 2022 "edicao_pasta" => "anais/colaob/2021" "edicao_logo" => null "edicao_capa" => "61fc15636d9b1_03022022144819.jpg" "data_publicacao" => "2022-02-21" "edicao_publicada_em" => "2022-01-26 10:43:13" "publicacao_id" => 91 "publicacao_nome" => "Revista do Latin American Congresso of Artificial Organs and Biomaterials (COLAOB)" "publicacao_codigo" => "978-65-86901-54-2" "tipo_codigo_id" => 2 "tipo_codigo_nome" => "ISBN" "tipo_publicacao_id" => 1 "tipo_publicacao_nome" => "ANAIS de Evento" ] #original: array:35 [ "id" => 83363 "edicao_id" => 195 "trabalho_id" => 88 "inscrito_id" => 217 "titulo" => "GALANTAMINE MICROPARTICLES REDUCE INFLAMMATION AND OXIDATIVE STRESS AFTER ACUTE SPINAL CORD INJURY IN RATS" "resumo" => """ SPINAL CORD INJURY (SCI) IS A DEBILITATING CONDITION WITH NO EFFECTIVE TREATMENT. ONE OF THE GREATEST DIFFICULTIES FOR TREATMENT ARISES FROM THE INFLAMMATION PROCESS. PREVIOUS STUDIES FROM OUR GROUP HAVE INDICATED THAT GALANTAMINE IMPROVES RECOVERY AFTER SCI, AND LITERATURE SHOWS ITS ANTI-INFLAMMATORY AND ANTIOXIDANT EFFECTS, AS WELL AS ITS NEUROPROTECTIVE PROPERTIES. POLYMERIC NANOPARTICLES, DUE TO THEIR DIMINISHED SIDE EFFECTS AND IMPROVED DRUG RELEASE CHARACTERISTICS, PRESENT A GOOD POSSIBILITY FOR TREATMENT. THIS STUDY AIMS TO USE NANOPARTICLES LOADED WITH GALANTAMINE TO MITIGATE SCI CONSEQUENCES IN TERMS OF INFLAMMATION AND OXIDATIVE STRESS. THE MICROPARTICLES WERE ELECTROSPUN WITH 2.5% OF GALANTAMINE IN A 4% PLGA SOLUTION OR 4% PLGA ALONE. THE \r\n DIAMETER AND ZETA POTENTIAL OF THE PARTICLES WERE MEASURED BY DYNAMIC LIGHT SCATTERING. WISTAR RATS WERE SUBMITTED TO A CONTUSION INJURY AT THE THORACIC SPINAL CORD (CEUA 35781). THE ANIMALS WERE DIVIDED INTO 5 GROUPS: LAMINECTOMY; SCI; SCI WITH GALANTAMINE; SCI WITH PLGA PARTICLES; AND SCI WITH PLGA-GALANTAMINE PARTICLES (PG). THREE DAYS AFTER THE INJURY, THE ANIMALS WERE EUTHANIZED AND THE SPINAL CORDS WERE COLLECTED. THE LEVELS OF IL-1β AND IL-6 WERE EVALUATED BY ELISA. THE PRODUCTION OF REACTIVE OXYGEN SPECIES (ROS) WAS ASSESSED BY MEASURING THE OXIDATION OF 2€², 7€²-DICHLOROFLUORESCIN DIACETATE (DCF) AND LEVELS OF LIPID PEROXIDATION WERE ANALYZED BY MEASURING THIOBARBITURIC ACID REACTIVE SUBSTANCES (TBARS). THE AVERAGE PARTICLE DIAMETER WAS 3247,6±1490,4NM FOR THE 4% PLGA PARTICLES AND 568,3±199,2NM FOR THE PG PARTICLES. THE ZETA POTENTIAL OF THE PARTICLES WAS -50,05±11.89MV FOR THE 4% PLGA PARTICLES AND -23,59±5.27MV FOR THE PG PARTICLES. THE GROUP TREATED WITH PG SHOWED SIGNIFICANTLY DECREASED IL-1β LEVELS WHEN COMPARED TO THE INJURY GROUP. INTERESTINGLY, THE PLGA GROUP PRESENTED DECREASED IL-6 LEVELS COMPARED TO THE INJURY AND LAMINECTOMY GROUPS. HOWEVER, ONLY THE ANIMALS TREATED WITH PG SHOWED DECREASED ROS PRODUCTION, WHEREAS THE PG AND GALANTAMINE GROUPS SHOWED DECREASED LEVELS OF LIPID PEROXIDATION. THUS, THE USE OF PG PARTICLES IS A PROMISING APPROACH FOR SPINAL CORD INJURY REGENERATION, CONSIDERING ITS EFFECTS IN BOTH THE DECREASE OF INFLAMMATORY CYTOKINES AND OXIDATIVE STRESS MARKERS. """ "modalidade" => "Poster" "area_tematica" => "NANOTECHNOLOGY IN BIOMATERIALS" "palavra_chave" => "SPINAL CORD INJURY, NANOTECHNOLOGY, GALANTAMINE, , " "idioma" => "Português" "arquivo" => "TRABALHO_EV170_MD4_SA_ID217_07122021114225.pdf" "created_at" => "2022-02-16 10:17:52" "updated_at" => null "ativo" => 1 "autor_nome" => "FERNANDA STAPENHORST FRANÇA" "autor_nome_curto" => "STAPENHORST FRANÇA" "autor_email" => "fernanda.stapenhorst@gmai" "autor_ies" => "UFRGS" "autor_imagem" => "" "edicao_url" => "anais-do-latin-american-congress-of-artificial-organs-and-biomaterials" "edicao_nome" => "Anais do XI Latin American Congress of Artificial Organs and Biomaterials (COLAOB)" "edicao_evento" => "XI Latin American Congress of Artificial Organs and Biomaterials" "edicao_ano" => 2022 "edicao_pasta" => "anais/colaob/2021" "edicao_logo" => null "edicao_capa" => "61fc15636d9b1_03022022144819.jpg" "data_publicacao" => "2022-02-21" "edicao_publicada_em" => "2022-01-26 10:43:13" "publicacao_id" => 91 "publicacao_nome" => "Revista do Latin American Congresso of Artificial Organs and Biomaterials (COLAOB)" "publicacao_codigo" => "978-65-86901-54-2" "tipo_codigo_id" => 2 "tipo_codigo_nome" => "ISBN" "tipo_publicacao_id" => 1 "tipo_publicacao_nome" => "ANAIS de Evento" ] #changes: [] #casts: array:14 [ "id" => "integer" "edicao_id" => "integer" "trabalho_id" => "integer" "inscrito_id" => "integer" "titulo" => "string" "resumo" => "string" "modalidade" => "string" "area_tematica" => "string" "palavra_chave" => "string" "idioma" => "string" "arquivo" => "string" "created_at" => "datetime" "updated_at" => "datetime" "ativo" => "boolean" ] #classCastCache: [] #attributeCastCache: [] #dates: [] #dateFormat: null #appends: [] #dispatchesEvents: [] #observables: [] #relations: [] #touches: [] +timestamps: false #hidden: [] #visible: [] +fillable: array:13 [ 0 => "edicao_id" 1 => "trabalho_id" 2 => "inscrito_id" 3 => "titulo" 4 => "resumo" 5 => "modalidade" 6 => "area_tematica" 7 => "palavra_chave" 8 => "idioma" 9 => "arquivo" 10 => "created_at" 11 => "updated_at" 12 => "ativo" ] #guarded: array:1 [ 0 => "*" ] }