PROTECTOR EFFECT OF BLACKCURRANT (RIBES NIGRUM L.) IN SCOPOLAMINE-INDUCED AMNESIA IN MICE
NAIARA STEFANELLO, PAULINE DA COSTA, CHARLES ELIAS ASSMANN e orientado por VERA MARIA MELCHIORS MORSCH e orientado por VERA MARIA MELCHIORS MORSCH
Alzheimer's disease (AD) is a multifactorial progressive neurodegenerative dysfunction that leads to deterioration of memory and other cognitive functions. In addition, studies have related changes in neurogenesis, cholinergic system and oxidative stress with the progression and development of AD. Thus, the search for compounds that prevent the cognitive damages caused by this disease becomes important. In this context, Blackcourrant (Ribes nigrum L.) has a high content of flavonoids, which may have beneficial effects on memory, since it has antioxidant and neuroprotective potential. Therefore, the objective of this project was to evaluate the mechanisms involved in the Blackcourrant (BC) neuroprotective action in a model of amnesia induced by scopolamine in mice. Male Swiss mice (n = 68) were be divided into 8 groups: Group 1: Control: oral saline i.p (n = 10) Group 2: Blackcourrant 50 mg/kg/saline i.p (n = 10); Group 3: Donepezil 5 mg/kg/saline i.p (n = 5); Group 4: Blackcourrant 50 mg/kg/Donepezil 5 mg/kg/saline i.p (n = 5); Group 5: Scopolamine 1 mg/kg/oral saline (n = 10); Group 6: Blackcurrant 50 mg/kg/Scopolamine 1 mg/kg (n = 10); Group 7: Donepezil 5 mg/kg/Scopolamine 1 mg (n = 10); Group 8: Blackcurrant 50 mg/kg/Donepezil 5 mg/kg/Scopolamine 1 mg/kg (n = 8), in which they will receive BC and/or Donepezil and/or Scopolamine for 28 days. The behavioral parameters, besides the acetylcholinesterase and butyrylcholinesterase activity were analyzed. The reactive species levels were also evaluated. The analyzes were performed in hippocampus and cerebral cortex. The neuroprotective effect of Blackcourant alone or in combination with Donepezil has been observed and it demonstrate improving cognitive deficits in animals submitted to amnesia, which makes this compound a possible therapeutic agent in the prevention of neurodegenerative diseases, such as AD.
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